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KMID : 0613820100200060902
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Journal of Life Science
2010 Volume.20 No. 6 p.902 ~ p.906
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Culture Conditions of Geobacillus kaustophilus DSM 7263 for Production of Thermophilic Extracellular Lipase
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Jeon Sung-Jong
Kang Hyun-Woo
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Abstract
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A producer of thermophilic extracellular lipase, Geobacillus kaustophilus DSM 7263, was selected from various microorganisms of the Geobacillus genus. We investigated optimum conditions for mass production of G. kaustophilus lipase. Among the different natural oil media, olive oil was optimal for enzyme production. The maximum amount of enzyme production was obtained when G. kaustophilus was grown in a medium containing 0.5% olive oil as a carbon source. The pH and temperature for optimal growth were pH 8.0 and 55℃, respectively, while the optimum pH and temperature for lipase production were pH 6.0 and 50℃, respectively. In the presence of Mg2+ and Mn2+, lipase production was dramatically enhanced by 247% and 157%, respectively, whereas enzyme production was inhibited by Zn2+, Cu2+, and Cd2+. The addition of 0.1% (v/v) triton X-100 increased lipase production and cell growth when compared to the negative control.
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KEYWORD
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Lipase, Geobacillus kaustophilus, thermophilic, extracellular, olive oil, triton X-100
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